Abstract— Multiple access interference (MAI) is one of the most important issues to consider in the design, implementation and operation of 5G systems using code division multiple access (CDMA) technique based on spread spectrum that offers a more flexible solution, compared to other techniques. Especially, it permits a much higher user bit rate, due to the bandwidth allocated to the emitted signal; thus, allowing very attractive multimedia services. In this paper, we develop the expressions of the detection probability, false alarm probability and the average acquisition time – in a direct sequence CDMA (DS-CDMA) transmission system – using a serial search and taking into consideration MAI. The evolution of these parameters according to the signal- to-noise ratio (SNR) and the decision threshold leads to study performances of the system in terms of detection and average acquisition time. The obtained results reveal that the acquisition time decreases when the SNR increases. Additionally, it is found that the probability of detection increases when the threshold decreases, and increasing SNR it reaches a constant value for an SNR/chip around 0 dB for all thresholds.